7EEH
Selenomethionine labeled Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TqaL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.746, 99.488, 193.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.480 - 2.000 |
| R-factor | 0.2328 |
| Rwork | 0.232 |
| R-free | 0.26090 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.137 | 1.527 |
| Number of reflections | 104375 | 4910 |
| <I/σ(I)> | 11.3 | 1.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 15.2 | |
| CC(1/2) | 0.999 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 293 | 100 mM Tris-HCl (pH 7.75), 0.2 M MgCl2, 10% PEG 8000 |
