7EEF
Crystal structure of EphA7 mutant G656E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-11-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 65 |
| Unit cell lengths | 51.360, 51.360, 215.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.479 - 2.600 |
| Rwork | 0.244 |
| R-free | 0.27970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rei |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.621 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.790 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | |
| Number of reflections | 9920 | 1450 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 1 | |
| CC(1/2) | 0.997 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | PEG3350, Ammonium sulphate, HEPES |
