7EEF

Crystal structure of EphA7 mutant G656E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-11-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54184
Spacegroup name	P 65
Unit cell lengths	51.360, 51.360, 215.172
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.479 - 2.600
Rwork	0.244
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rei
RMSD bond length	0.009
RMSD bond angle	1.621
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.790	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.120
Number of reflections	9920	1450
<I/σ(I)>	15.9
Completeness [%]	99.9
Redundancy	1
CC(1/2)	0.997	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	PEG3350, Ammonium sulphate, HEPES