7EED
Crystal structure of EphA7 mutant D751H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-01-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 65 |
| Unit cell lengths | 51.240, 51.240, 215.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.375 - 3.050 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rei |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.680 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.760 | 3.220 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.185 | |
| Number of reflections | 5689 | 768 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 92.5 | |
| Redundancy | 1 | |
| CC(1/2) | 0.988 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | PEG3350, Ammonium sulphate, HEPES |
