7EEA
Cyanophage Pam1 tailspike receptor-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 123.580, 241.272, 176.429 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.439 - 2.671 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.25460 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.671 | 2.671 |
Rmerge | 0.857 | |
Number of reflections | 72867 | 72556 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 98.5 | |
Redundancy | 12.3 | |
CC(1/2) | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 1.34M sodium phosphate monobasic monohydrate, 0.06M potassium phosphate dibasic |