7EE3
Crystal structure of PltC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 189.607, 189.607, 41.206 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.790 - 1.330 |
R-factor | 0.1621 |
Rwork | 0.161 |
R-free | 0.18430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wht |
RMSD bond length | 0.016 |
RMSD bond angle | 1.509 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.790 | 1.378 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmeas | 0.059 | 0.384 |
Number of reflections | 124886 | 11420 |
<I/σ(I)> | 32.45 | |
Completeness [%] | 98.3 | |
Redundancy | 4.8 | |
CC(1/2) | 0.995 | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 34% PEG500MME and 0.1M sodium citrate |