7EDU
RNA duplex containing C-Au-C base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03906 |
Spacegroup name | P 61 |
Unit cell lengths | 29.216, 29.216, 257.794 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.180 - 2.300 |
R-factor | 0.1819 |
Rwork | 0.177 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RNA duplex constructed using Coot |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.180 | 25.180 | 2.360 |
High resolution limit [Å] | 2.300 | 10.280 | 2.300 |
Rmerge | 0.057 | 0.020 | 0.316 |
Rmeas | 0.073 | 0.024 | 0.394 |
Total number of observations | 26576 | ||
Number of reflections | 10301 | 110 | 738 |
<I/σ(I)> | 11.1 | 24.03 | 2.73 |
Completeness [%] | 94.1 | 94 | 93.5 |
Redundancy | 2.58 | 2.864 | 2.638 |
CC(1/2) | 0.997 | 0.999 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, MPD, spermine tetrahydrochloride, ammonium chloride, gold chloride |