7EDM

Crystal structure of the eukaryotic ribosomal decoding site in complex with G418 and Hg(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	31.429, 97.487, 47.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 2.810
R-factor	0.1702
Rwork	0.164
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k32
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.740	48.740	2.890
High resolution limit [Å]	2.810	12.580	2.810
Rmerge	0.099	0.056	0.332
Rmeas	0.117	0.068	0.389
Total number of observations	24046
Number of reflections	6827	79	493
<I/σ(I)>	9.86	18.16	3.74
Completeness [%]	99.5	98.8	97.6
Redundancy	3.522	3.076	3.458
CC(1/2)	0.992	0.992	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, MPD, spermine tetrahydrochloride, potassium chloride, G418, mercury(II) perchlorate