7EDL
Crystal structure of the bacterial ribosomal decoding site in complex with G418 and Hg(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-23 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 31.640, 97.301, 47.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.650 - 2.600 |
R-factor | 0.1899 |
Rwork | 0.186 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k32 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.650 | 48.650 | 2.670 |
High resolution limit [Å] | 2.600 | 11.650 | 2.610 |
Rmerge | 0.055 | 0.030 | 0.336 |
Rmeas | 0.065 | 0.035 | 0.395 |
Number of reflections | 8525 | 93 | 631 |
<I/σ(I)> | 15.16 | 29.54 | 3.42 |
Completeness [%] | 99.3 | 95.9 | 99.2 |
Redundancy | 3.516 | 3.14 | 3.593 |
CC(1/2) | 0.998 | 0.998 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, MPD, sodium chloride, spermine tetrahydrochloride, G418, mercury(II) perchlorate |