7EDB

EcoT38I restriction endonuclease complexed with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	173
Detector technology	CCD
Collection date	2011-02-08
Detector	MARRESEARCH
Wavelength(s)	0.9000
Spacegroup name	P 64 2 2
Unit cell lengths	156.291, 156.291, 173.049
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.410 - 2.390
R-factor	0.1636
Rwork	0.162
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e8r
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.410	2.540
High resolution limit [Å]	2.390	2.390
Rmerge	0.082	0.571
Rmeas	0.085	0.595
Number of reflections	49667	7888
<I/σ(I)>	26.24	6.07
Completeness [%]	99.9	99.9
Redundancy	12.15	12.15
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	28% PEG400 0.2 M Calcium chloride 0.1 M Potassium chloride 0.05 M Tris-HCl