7ECQ
Crystal structure of FAM3A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.968 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.186, 60.782, 78.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.453 - 1.381 |
R-factor | 0.1636 |
Rwork | 0.163 |
R-free | 0.17130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yoq |
Data reduction software | Adxv |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.055 | 0.394 |
Number of reflections | 39780 | 3768 |
<I/σ(I)> | 44.35 | |
Completeness [%] | 95.4 | |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289.15 | 0.2 M Ammonium Sulfate, 0.1 M MES pH6.5, 30% w/v Polyethylene Glycol Monomethyl Ether 5000 |