7ECP
RNA duplex containing C-Ag-U
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-22 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | I 4 |
Unit cell lengths | 88.968, 88.968, 33.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.450 - 2.910 |
R-factor | 0.1608 |
Rwork | 0.154 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.450 | 31.450 | 2.990 |
High resolution limit [Å] | 2.910 | 13.030 | 2.910 |
Rmerge | 0.094 | 0.039 | 0.204 |
Rmeas | 0.110 | 0.047 | 0.236 |
Total number of observations | 10945 | ||
Number of reflections | 2968 | 37 | 223 |
<I/σ(I)> | 9.9 | 20.44 | 4.47 |
Completeness [%] | 99.3 | 92.5 | 98.2 |
Redundancy | 3.688 | 2.892 | 3.646 |
CC(1/2) | 0.995 | 0.999 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MOPS, MPD, spermine, lithium nitrate |