7ECO
RNA duplex containing U-Ag-U base pairs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.771, 26.875, 78.944 |
Unit cell angles | 90.00, 101.69, 90.00 |
Refinement procedure
Resolution | 36.910 - 1.810 |
R-factor | 0.1882 |
Rwork | 0.185 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.910 | 36.910 | 1.860 |
High resolution limit [Å] | 1.810 | 8.090 | 1.810 |
Rmerge | 0.059 | 0.040 | 0.388 |
Rmeas | 0.064 | 0.044 | 0.425 |
Total number of observations | 118980 | ||
Number of reflections | 18483 | 236 | 1350 |
<I/σ(I)> | 17.77 | 32.81 | 5.16 |
Completeness [%] | 99.7 | 97.5 | 99.2 |
Redundancy | 6.437 | 5.75 | 6.054 |
CC(1/2) | 0.999 | 0.999 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MOPS, MPD, spermine, lithium nitrate |