7ECN
RNA duplex containing C-Ag-A base pairs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.784, 41.482, 45.934 |
Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
Resolution | 34.970 - 1.600 |
R-factor | 0.173 |
Rwork | 0.170 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.970 | 34.970 | 1.640 |
High resolution limit [Å] | 1.600 | 7.170 | 1.600 |
Rmerge | 0.040 | 0.019 | 0.291 |
Rmeas | 0.048 | 0.023 | 0.350 |
Total number of observations | 56696 | ||
Number of reflections | 17296 | 206 | 1261 |
<I/σ(I)> | 16.46 | 40.23 | 3.86 |
Completeness [%] | 99.1 | 97.2 | 98.1 |
Redundancy | 3.278 | 3.107 | 3.206 |
CC(1/2) | 0.999 | 1.000 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MOPS, MPD, spermine, potassium nitrate |