7ECM
RNA duplex containing C-A base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-22 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 |
Unit cell lengths | 26.475, 27.909, 46.665 |
Unit cell angles | 100.08, 93.54, 110.47 |
Refinement procedure
Resolution | 45.540 - 1.510 |
R-factor | 0.1611 |
Rwork | 0.157 |
R-free | 0.19550 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.540 | 45.540 | 1.550 |
High resolution limit [Å] | 1.510 | 6.740 | 1.510 |
Rmerge | 0.057 | 0.050 | 0.276 |
Rmeas | 0.067 | 0.060 | 0.323 |
Number of reflections | 18436 | 210 | 1348 |
<I/σ(I)> | 13.37 | 22.97 | 5.51 |
Completeness [%] | 95.4 | 96.8 | 91.1 |
Redundancy | 3.635 | 3.4 | 3.711 |
CC(1/2) | 0.998 | 0.995 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MOPS, MPD, spermine, barium nitrate |