7ECK

RNA duplex containing C-Ag-A and U-Ag-A base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	43.408, 43.408, 379.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.190 - 2.790
R-factor	0.2275
Rwork	0.220
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.190	42.190	2.860
High resolution limit [Å]	2.790	12.480	2.790
Rmerge	0.060	0.030	0.332
Rmeas	0.069	0.038	0.373
Total number of observations	50030
Number of reflections	11225	160	769
<I/σ(I)>	14.1	24.56	3.92
Completeness [%]	98.9	93.6	94.2
Redundancy	4.457	3.362	4.559
CC(1/2)	0.999	0.999	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, lithium nitrate