7ECJ

RNA duplex containing C-A base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 63 2 2
Unit cell lengths	47.780, 47.780, 186.215
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.550 - 3.100
R-factor	0.2364
Rwork	0.231
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.550	46.550	3.180
High resolution limit [Å]	3.100	13.880	3.100
Rmerge	0.070	0.030	0.415
Rmeas	0.075	0.034	0.439
Total number of observations	22964
Number of reflections	2674	46	202
<I/σ(I)>	21.51	44.63	5.14
Completeness [%]	99.7	97.9	99
Redundancy	8.588	4.5	9.475
CC(1/2)	0.999	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, sodium cacodylate, spermine tetrahydrochloride, lithium nitrate