7EC7

Crystal structure of SdgB (complexed with phosphate ions)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	287
Detector technology	CCD
Collection date	2014-02-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	172.257, 172.257, 106.174
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.980 - 3.200
R-factor	0.1993
Rwork	0.196
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ec1
RMSD bond length	0.004
RMSD bond angle	1.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	26949	1492
<I/σ(I)>	16.9
Completeness [%]	89.0
Redundancy	8.2
CC(1/2)	0.973	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	1.2 M NaH2PO4, 0.8 M K2HPO4, and 0.1 M CAPS-NaOH (pH 10.5)