7EC0
Crystal structure of juvenile hormone acid methyltransferase JHAMT in complex with S-Adenosyl homocysteine and methyl farnesoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.459, 101.203, 103.363 |
| Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
| Resolution | 72.260 - 2.494 |
| Rwork | 0.245 |
| R-free | 0.29260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ebx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.260 | 2.630 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.056 | 0.734 |
| Rmeas | 0.062 | 0.792 |
| Number of reflections | 58792 | 8516 |
| <I/σ(I)> | 18.2 | 0.6 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.15M DL-Malic acid pH 7.0, 20% PEG 3350 |
