7EBY
Crystal structure of D-Succinylase (DSA) from Cupriavidus sp. P4-10-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-10 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 83.586, 92.117, 162.111 |
Unit cell angles | 94.79, 96.31, 104.90 |
Refinement procedure
Resolution | 47.630 - 2.000 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4HST.pdb |
RMSD bond length | 0.007 |
RMSD bond angle | 0.935 |
Data reduction software | DENZO |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.120 | 0.602 |
Rpim | 0.076 | 0.379 |
Number of reflections | 303340 | 15023 |
<I/σ(I)> | 7.5 | 2.4 |
Completeness [%] | 97.8 | 96.8 |
Redundancy | 2.5 | 2.4 |
CC(1/2) | 0.991 | 0.663 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 24% PEG 1500, 20% Glycerol |