7EBX

Crystal structure of juvenile hormone acid methyltransferase JHAMT in complex with S-adenosyl-L-homocysteine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 43 2 2
Unit cell lengths	102.155, 102.155, 102.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.690 - 2.890
Rwork	0.251
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ebs
RMSD bond length	0.003
RMSD bond angle	1.224
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.694	2.950
High resolution limit [Å]	2.890	2.890
Rmerge	0.082	0.908
Number of reflections	12618	603
<I/σ(I)>	42.867	1.795
Completeness [%]	100.0	100
Redundancy	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1M Bis-Tris pH 5.5, 1M ammonium sulfate, 1% PEG 3350