7EBT
Crystal structure of Aedes aegypti Noppera-bo, glutathione S-transferase epsilon 8, in glutathione-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 151.877, 151.877, 147.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.030 - 1.510 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kem |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.030 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rpim | 0.022 | 0.388 |
| Number of reflections | 132068 | 6411 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 99.1 | |
| Redundancy | 27.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 32.5% PEG 4000, 500mM CaCl2, 100mM Tris-HCl pH 7.5 |
