7EBS
Crystal structure of juvenile hormone acid methyltransferase JHAMT from silkworm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 99.584, 99.584, 70.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.240 - 2.950 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28120 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.240 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.048 | 0.660 |
| Rmeas | 0.052 | 0.711 |
| Number of reflections | 8562 | 506 |
| <I/σ(I)> | 30.66 | 2.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M Bis-Tris pH 5.5, 1M ammonium sulfate, 1% PEG 3350 |
