7EBJ
Apo structure of the mouse Trim66 PHD-Bromo dual domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2017-03-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97780 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.073, 80.073, 80.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.220 - 1.800 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o33 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.855 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 24555 | 1208 |
<I/σ(I)> | 24.8 | |
Completeness [%] | 99.5 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20000 |