7EB9
The structure of the A20-binding inhibitor of NF-kB 1 in complex with tetra-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2014-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9766 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.381, 53.777, 240.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 3.200 |
| R-factor | 0.2211 |
| Rwork | 0.216 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eao |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.089 | 0.541 |
| Number of reflections | 10149 | 790 |
| <I/σ(I)> | 16.4 | 3.47 |
| Completeness [%] | 96.3 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Sodium malonate pH 6.0, 10% PEG 3350 |
