7EB6
Crystal structure of GTP-binding protein-like domain of AGAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 4 |
| Unit cell lengths | 100.390, 100.390, 48.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.493 - 3.014 |
| R-factor | 0.2359 |
| Rwork | 0.233 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iwr |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.493 | 3.122 |
| High resolution limit [Å] | 3.014 | 3.014 |
| Rmerge | 0.130 | |
| Number of reflections | 9136 | 458 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.949 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | PEG 8000 |
