7EAP

Crystal structure of IpeA-XXXG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	PIXEL
Collection date	2018-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 31 2 1
Unit cell lengths	94.197, 94.197, 189.003
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.480 - 1.420
R-factor	0.1195
Rwork	0.118
R-free	0.14690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yot
RMSD bond length	0.017
RMSD bond angle	2.110
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.120	1.470
High resolution limit [Å]	1.420	1.420
Number of reflections	181988	17579
<I/σ(I)>	10.4
Completeness [%]	99.7
Redundancy	10.1
CC(1/2)	0.995	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris pH 5.5, 2.0 M Ammonium sulfate