7EAF
Crystal structure of SAM-I riboswitch with the Actinomyces-1 k-turn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.460, 61.460, 157.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.220 - 2.850 |
R-factor | 0.2639 |
Rwork | 0.262 |
R-free | 0.29760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fk3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.965 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.154 | |
Rpim | 0.056 | 0.656 |
Number of reflections | 7963 | 1123 |
<I/σ(I)> | 8.9 | 1.4 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 7.2 | 7.7 |
CC(1/2) | 0.990 | 0.771 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 40 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD. |