7EA7

crystal structure of NAP1 LIR in complex with GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	35.096, 73.584, 103.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.840 - 2.690
R-factor	0.2141
Rwork	0.211
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bv4
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.790	2.820
High resolution limit [Å]	2.690	2.690
Number of reflections	7940	1025
<I/σ(I)>	21.9
Completeness [%]	100.0
Redundancy	12.4
CC(1/2)	0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.3 M sodium nitrate, 0.3 M sodium phosphate dibasic, 0.3 M ammonium sulfate, 0.1 M bicine-tris pH 8.5, 25% v/v MPD, 25% PEG1000, 25% w/v PEG3350