7EA7
crystal structure of NAP1 LIR in complex with GABARAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.096, 73.584, 103.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.840 - 2.690 |
R-factor | 0.2141 |
Rwork | 0.211 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bv4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.790 | 2.820 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 7940 | 1025 |
<I/σ(I)> | 21.9 | |
Completeness [%] | 100.0 | |
Redundancy | 12.4 | |
CC(1/2) | 0.999 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.3 M sodium nitrate, 0.3 M sodium phosphate dibasic, 0.3 M ammonium sulfate, 0.1 M bicine-tris pH 8.5, 25% v/v MPD, 25% PEG1000, 25% w/v PEG3350 |