7EA4

Crystal Structure of L182E D-Succinylase (DSA) from Cupriavidus sp. P4-10-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-06-14
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	62.057, 129.806, 200.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.930 - 1.950
R-factor	0.1666
Rwork	0.164
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hst
RMSD bond length	0.007
RMSD bond angle	0.881
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.112
Rmeas	0.112	0.619
Rpim	0.051	0.284
Number of reflections	118124	5812
<I/σ(I)>	11
Completeness [%]	99.6	99.9
Redundancy	4.7	4.5
CC(1/2)	0.993	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	11% PEG 8000, 0.1M Sodium cacodylate, 0.2M MaCl2