7EA2

crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	67.620, 67.620, 247.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.560 - 2.140
R-factor	0.2026
Rwork	0.201
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7czg
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	247.370	2.200
High resolution limit [Å]	2.140	2.140
Number of reflections	19420	1384
<I/σ(I)>	16
Completeness [%]	99.7
Redundancy	37.4
CC(1/2)	0.997	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M phosphate/citrate pH 4.2, 40% PEG300