7EA2
crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 67.620, 67.620, 247.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.560 - 2.140 |
R-factor | 0.2026 |
Rwork | 0.201 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7czg |
RMSD bond length | 0.007 |
RMSD bond angle | 0.891 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 247.370 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 19420 | 1384 |
<I/σ(I)> | 16 | |
Completeness [%] | 99.7 | |
Redundancy | 37.4 | |
CC(1/2) | 0.997 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M phosphate/citrate pH 4.2, 40% PEG300 |