7EA1
Crystal Structure of Spindlin1 bound to SPINDOC Docpep2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.795, 136.904, 42.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.363 - 2.700 |
R-factor | 0.2553 |
Rwork | 0.254 |
R-free | 0.28590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ns2 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.689 | 7.320 | 2.700 |
Rmerge | 0.103 | 0.084 | 0.422 |
Rmeas | 0.113 | 0.093 | 0.465 |
Rpim | 0.045 | 0.038 | 0.192 |
Number of reflections | 14134 | 799 | 671 |
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.8 | 99 | 99.9 |
Redundancy | 6.4 | 5.6 | 5.6 |
CC(1/2) | 0.993 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 0.1M Sodium chloride, 0.1M BIS-TRIS pH 6.5, 1.5M Ammonium sulfate |