7E9X
Trehalase of Arabidopsis thaliana acid mutant -D380A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-19 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.657, 129.647, 151.141 |
Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
Resolution | 41.550 - 1.880 |
R-factor | 0.1938 |
Rwork | 0.193 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e9u |
RMSD bond length | 0.007 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.550 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 169902 | 27135 |
<I/σ(I)> | 14.34 | |
Completeness [%] | 99.8 | |
Redundancy | 6.82 | |
CC(1/2) | 0.999 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mM Tre6P, 0.1 M sodium chloride and 20% PEG3400. |