7E9U

Trehalase of Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.981, 129.813, 155.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.218 - 2.100
R-factor	0.2105
Rwork	0.209
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jg0
RMSD bond length	0.008
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	66029	10391
<I/σ(I)>	17.35
Completeness [%]	99.7	98.8
Redundancy	7.4	7.4
CC(1/2)	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate and 20% PEG3400