7E92
Crystal structure of the DNA-binding domain of the response regulator VbrR from Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.315, 51.758, 97.224 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.1882 |
Rwork | 0.186 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.728 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.054 | 0.490 |
Number of reflections | 17222 | 838 |
<I/σ(I)> | 33.7 | 3.5 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 400, MES, PEG 3350 | |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 400, MES, PEG 3350 |