7E90

Crystal structure of the receiver domain (D51E) of the response regulator VbrR from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	74.128, 74.128, 108.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.2346
Rwork	0.233
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zwm
RMSD bond length	0.004
RMSD bond angle	0.804
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.088	0.546
Number of reflections	14862	708
<I/σ(I)>	21.4
Completeness [%]	99.7	98.7
Redundancy	5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 400, Tris, lithium sulfate