7E8R
EcoT38I restriction endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.159, 53.159, 250.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.200 - 1.900 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.24130 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.735 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | RESOLVE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.460 |
| Rmeas | 0.059 | 0.400 |
| Number of reflections | 33635 | 5245 |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 10.4 | 10.4 |
| CC(1/2) | 0.999 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 | 10% PEG6000, 5% MPD, 0.1M HEPES |
