7E8O

Crystal structure of proteinaceous RNase P(PRORP) from Planctomycetes bacterium GWF2_40_8 complexed with Escherichia coli histidine pre-tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2019-10-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	146.277, 146.277, 143.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.459 - 2.804
R-factor	0.2323
Rwork	0.230
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e8k
RMSD bond length	0.009
RMSD bond angle	1.604
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.117	0.035	0.592
Rmeas	0.121	0.036	0.632
Rpim	0.030	0.008	0.216
Total number of observations	584223
Number of reflections	38678	4128	3763
<I/σ(I)>	6.9
Completeness [%]	99.9	99.8	99.8
Redundancy	15.1	21.7	8
CC(1/2)		0.998	0.053

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8% (v/v) Tacsimate pH 8.0, 20% (w/v) PEG 3350