7E8K
Crystal structure of Proteinaceous RNase P (PRORP) from Planctomycetes bacterium GWF2_40_8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-11-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 |
Unit cell lengths | 99.932, 99.932, 176.178 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.363 - 2.250 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e8j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.330 |
High resolution limit [Å] | 2.249 | 4.840 | 2.250 |
Rmerge | 0.093 | 0.051 | 0.361 |
Rmeas | 0.098 | 0.054 | 0.410 |
Rpim | 0.033 | 0.017 | 0.188 |
Number of reflections | 92631 | 9337 | 8688 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 99.0 | 99.8 | 92.9 |
Redundancy | 7.1 | 9.7 | 3.8 |
CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2000mM Ammonium sulfate, 100mM Sodium citrate/Citric acid pH 5.5 |