7E8J

Crystal structure of Proteinaceous RNase P (PRORP) from Thermococcus celer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	33.276, 108.694, 115.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.527 - 2.100
R-factor	0.2124
Rwork	0.211
R-free	0.23080
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.941
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.095	5.700	2.100
Rmerge	0.087	0.048	0.374
Rmeas	0.092	0.050	0.453
Rpim	0.029	0.015	0.249
Number of reflections	24758	1407	984
<I/σ(I)>	6.3
Completeness [%]	97.0	98.4	79.9
Redundancy	7.8	10.9	2.3
CC(1/2)		0.999	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% (w/v) PEG 3000, 100mM Tris-HCl pH 7.0, 200mM NaCl.