7E8J
Crystal structure of Proteinaceous RNase P (PRORP) from Thermococcus celer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.276, 108.694, 115.184 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.527 - 2.100 |
R-factor | 0.2124 |
Rwork | 0.211 |
R-free | 0.23080 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.941 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.095 | 5.700 | 2.100 |
Rmerge | 0.087 | 0.048 | 0.374 |
Rmeas | 0.092 | 0.050 | 0.453 |
Rpim | 0.029 | 0.015 | 0.249 |
Number of reflections | 24758 | 1407 | 984 |
<I/σ(I)> | 6.3 | ||
Completeness [%] | 97.0 | 98.4 | 79.9 |
Redundancy | 7.8 | 10.9 | 2.3 |
CC(1/2) | 0.999 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% (w/v) PEG 3000, 100mM Tris-HCl pH 7.0, 200mM NaCl. |