7E7X

SARS-CoV-2 Spike Protein N terminal domain in Complex with N11 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	91.423, 113.279, 210.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.470 - 2.780
R-factor	0.2495
Rwork	0.248
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c2l
RMSD bond length	0.007
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	2.879
High resolution limit [Å]	2.780	2.780
Number of reflections	55745	5095
<I/σ(I)>	15.17
Completeness [%]	98.7
Redundancy	13.1
CC(1/2)	0.994	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.2 M Magnesium chloride, 0.1 M Tris, pH 7.0, and 10% (w/v) polyethylene glycol 8000