7E7W
Crystal structure of RSL mutant in complex with sugar Ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 |
Unit cell lengths | 46.567, 46.554, 103.254 |
Unit cell angles | 90.04, 89.92, 119.98 |
Refinement procedure
Resolution | 26.200 - 4.100 |
R-factor | 0.2063 |
Rwork | 0.200 |
R-free | 0.34670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4csd |
Data reduction software | HKL-2000 |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 4.250 |
High resolution limit [Å] | 4.100 | 8.770 | 4.100 |
Rmerge | 0.120 | 0.038 | 0.400 |
Rmeas | 0.143 | 0.045 | 0.480 |
Rpim | 0.078 | 0.025 | 0.263 |
Total number of observations | 19061 | ||
Number of reflections | 6095 | 618 | 629 |
<I/σ(I)> | 4.7 | ||
Completeness [%] | 99.2 | 99.4 | 98.9 |
Redundancy | 3.1 | 3.1 | 3 |
CC(1/2) | 0.990 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 7.5 | 277 | 20 mM Tris-HCl, 100 mM of NaCl pH 7.5, the crystal was obtained in Micro centrifuge tube sequentially put with RSL solution, pure buffer, and the ligand solution |