7E7V
Crystal structure of RSL mutant in complex with sugar Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.494, 161.519, 54.137 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 80.760 - 1.610 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csd |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.960 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.760 | 80.760 | 1.690 |
| High resolution limit [Å] | 1.610 | 5.080 | 1.610 |
| Rmerge | 0.079 | 0.057 | 0.590 |
| Rmeas | 0.086 | 0.062 | 0.662 |
| Rpim | 0.034 | 0.024 | 0.296 |
| Total number of observations | 597512 | 21043 | 64788 |
| Number of reflections | 94580 | 3116 | 13552 |
| <I/σ(I)> | 15 | 32 | 2.7 |
| Completeness [%] | 98.0 | 99.8 | 96.7 |
| Redundancy | 6.3 | 6.8 | 4.8 |
| CC(1/2) | 0.998 | 0.998 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 277 | 20 mM Tris-HCl, 100 mM of NaCl pH 7.5, Micro centrifuge tube sequentially put with RSL solution, pure buffer, ligand solution |
