7E7U
Crystal structure of RSL mutant in complex with sugar Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.802, 79.470, 152.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.260 - 2.100 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.977 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.260 | 35.240 | 2.110 |
| High resolution limit [Å] | 2.010 | 6.340 | 2.010 |
| Rmerge | 0.136 | 0.046 | 1.316 |
| Rmeas | 0.142 | 0.049 | 1.371 |
| Rpim | 0.039 | 0.015 | 0.377 |
| Total number of observations | 15343 | 42350 | |
| Number of reflections | 37046 | 1402 | 3325 |
| <I/σ(I)> | 14 | 35.3 | 2.2 |
| Completeness [%] | 92.9 | 99 | 58.5 |
| Redundancy | 13 | 10.9 | 12.7 |
| CC(1/2) | 0.999 | 0.999 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 277 | 20 mM Tris-HCl, 100 mM of NaCl; pH 7.5, Micro centrifuge tube sequentially put with RSL solution, pure buffer, and the ligand solution |
