7E7T

Crystal structure of RSL mutant in complex with sugar Ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	44.342, 88.554, 67.893
Unit cell angles	90.00, 93.83, 90.00

Refinement procedure

Resolution	67.740 - 1.980
R-factor	0.2593
Rwork	0.257
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csd
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.740	67.740	2.080
High resolution limit [Å]	1.980	6.250	1.980
Rmerge	0.110	0.066	0.548
Rmeas	0.120	0.072	0.595
Rpim	0.047	0.028	0.230
Total number of observations	210690	7185	25842
Number of reflections	32990	1202	3978
<I/σ(I)>	9.2	17.3	3.1
Completeness [%]	89.6	99.5	74.5
Redundancy	6.4	6	6.5
CC(1/2)	0.996	0.996	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	7.5	277	20 mM Tris-HCl, 100 mM of NaCl; pH 7.5, Micro centrifuge tube sequentially put with RSL solution, pure b