7E7M

Crystal Structure Analysis of the Streptococcus agalactiae Ribose Binding Protein RbsB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	39.631, 151.220, 169.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.590 - 2.850
R-factor	0.2126
Rwork	0.210
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ioy
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.630	39.630	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.122	0.028	0.652
Rmeas	0.134	0.030	0.722
Rpim	0.055	0.012	0.301
Total number of observations	121350
Number of reflections	22672	674	3375
<I/σ(I)>	11.7
Completeness [%]	94.4	84.2	97.2
Redundancy	5.4	5.6	5.3
CC(1/2)	0.996	0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	jeffamime ED-2001