7E7H
Crystal Structure of a pseudooxynicotine amine oxidase Pnao from Pseudomonas putida S16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.657, 89.709, 157.583 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.830 - 2.200 |
R-factor | 0.1676 |
Rwork | 0.165 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c71 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.187 | 0.643 |
Rpim | 0.065 | 0.223 |
Number of reflections | 51082 | 2538 |
<I/σ(I)> | 18.5 | 4.5 |
Completeness [%] | 100.0 | |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Lithium sulfate, 25 % PEG 3350, BIS-TRIS ph 6.5 |