7E78

the structure of cytosolic TaPGI with substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	243.616, 74.449, 125.585
Unit cell angles	90.00, 94.12, 90.00

Refinement procedure

Resolution	35.680 - 2.210
R-factor	0.2335
Rwork	0.233
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ujh
RMSD bond length	0.008
RMSD bond angle	0.950
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.080
High resolution limit [Å]	2.040	2.040
Rmerge	0.088
Number of reflections	112442	14
<I/σ(I)>	7.2
Completeness [%]	99.3
Redundancy	3.9
CC(1/2)		0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	MES, PEG MME2000