7E77

The structure of cytosolic TaPGI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	244.317, 74.902, 125.338
Unit cell angles	90.00, 93.80, 90.00

Refinement procedure

Resolution	37.910 - 2.040
R-factor	0.2542
Rwork	0.254
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ujh
RMSD bond length	0.002
RMSD bond angle	0.524
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rpim	0.045
Number of reflections	132222	20
<I/σ(I)>	23.9
Completeness [%]	99.7
Redundancy	5.2
CC(1/2)		0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M NaCl, 0.1 M Bis-Tris pH 5.5, 25% (w/v) Polyethylene glycol 3350