7E6H

glucose-6-phosphate dehydrogenase from Kluyveromyces lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 65 2 2
Unit cell lengths	120.670, 120.670, 213.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.940 - 2.700
R-factor	0.1906
Rwork	0.187
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bh9
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	26093	2543
<I/σ(I)>	31.8
Completeness [%]	100.0
Redundancy	21.3
CC(1/2)	0.997	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium malonate dibasic at pH 5.5 and 20% (w/v) PEG 3350