7E59

interferon-inducible anti-viral protein truncated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 2 21 21
Unit cell lengths	90.874, 137.312, 203.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 3.000
R-factor	0.22
Rwork	0.218
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f5n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.127	0.049	1.883
Rmeas	0.135	0.052	1.999
Rpim	0.045	0.018	0.664
Total number of observations	475816
Number of reflections	52289	2835	2595
<I/σ(I)>	5.1
Completeness [%]	100.0	99.9	100
Redundancy	9.1	8.1	9
CC(1/2)		0.998	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		289.15	Tacsimate pH 5.0, Polyethylene glycol 3350